ARL8B regulates lysosomal function and predicts poor prognosis in hepatocellular carcinoma.

ABSTRACT: Adenosine 5'-diphosphate ribosylation factor-like 8B (ARL8B), a small GTPase, is involved in lysosome motility. Our study investigates the role of ARL8B in hepatocellular carcinoma (HCC) using in vitro and in vivo experiments, bioinformatics, and clinical data. We found that ARL8B expression is abnormally elevated in HCC and correlates with poor prognosis. ARL8B knockdown triggered lysosomal dysfunction-manifesting as abnormal morphology, decreased pH, reduced hydrolase activity, and impaired autophagic degradation-which subsequently led to cell cycle arrest and reduced cell viability. Additionally, tumors with high ARL8B expression (ARL8B^high) exhibited notable differences in tumor microenvironment composition compared to those with low ARL8B expression (ARL8B^low). A

SUBMITTER: Wu L 

PROVIDER: S-EPMC11985964 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature