Molecular evidence of Brucella abortus circulating in cattle, goats, and humans in Central Equatoria State, South Sudan.

ABSTRACT: Brucellosis is a neglected zoonotic disease in most developing countries, including South Sudan. Precise identification of Brucella species is crucial for addressing public health and epidemiological concerns associated with brucellosis. The study aimed to identify Brucella species using real-time polymerase chain reaction (qPCR) from seropositive samples that were acquired from an earlier investigation. A total of 143 genomic DNA samples were extracted from brucellosis Rose Bengal plate test (RBPT) seropositive samples from humans (n = 7), cattle (n = 103) and goats (n = 33). The samples were collected from Terekeka and Juba counties, Central Equatoria State (CES), South Sudan. The qPCR targeting the Brucella-specific IS711 insertion gene at the genus level was performed. Samples with a c

SUBMITTER: Lita EP 

PROVIDER: S-EPMC11986056 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature