Thiol isomerase ERp18 enhances platelet activation and arterial thrombosis.

ABSTRACT:

Background

Thiol isomerases regulate the thiol-disulfide exchange of functional proteins in cells. Using genetically modified mouse models and inhibitors, we and others demonstrated that 7 thiol isomerases (ERp57, protein diisulfide isomerase, ERp72, ERp46, ERp5, TMX4, and TMX1) participate in thrombosis. There are 21 thiol isomerases in mammals, but whether other enzymes of this family also contribute to thrombosis remains unknown.

Objectives

Investigate whether and how ERp18 participates in arterial thrombosis.

Methods

ERp18 knockout mice and arterial thrombosis models were used to determine the role of ERp18 in thrombosis. Platelets from ERp18 knockout mice were used to detect aggregation, activation, spreading, and clot retraction. Finally, flow cytometry and immu

SUBMITTER: He C 

PROVIDER: S-EPMC11986512 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature