<i>BrRCO</i> promotes leaf lobe formation by repressing <i>BrACP5</i> expression in <i>Brassica rapa</i>.

ABSTRACT: Lobed leaves are advantageous for gas exchange, canopy architecture, and high-density planting; however, the genetic mechanisms of leaf lobe formation in Brassica crops remains poorly understood. Here, lob10.1, our previously identified major QTL controlling the presence/absence of leaf lobes in B. rapa (AA), was fine mapped to a confidence interval of 69.8 kb. REDUCED COMPLEXITY ORGAN (BrRCO, BraA10g032440.3c), a homeodomain leucine zipper class I (HD ZIP I) transcription factor, was predicted to be the most likely candidate gene underlying lob10.1. Null mutations of BrRCO by CRISPR/Cas9 in the lobed-leaf parent RcBr and over-expression in the counter-part near isogenic lines (NIL^RcBr) lead to entire and lobed leaves, resp

SUBMITTER: Sun Y 

PROVIDER: S-EPMC11986587 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature