Application of Environmental DNA for Assessing the Distribution and Biomass of <i>Brachymystax lenok</i> Tsinlingensis in the Zhouzhi Heihe River.

ABSTRACT: In recent years, the population of Brachymystax lenok tsinlingensis has experienced a rapid decline due to habitat degradation caused by overfishing and dam construction. In the current study, water samples were collected from four tanks containing B. lenok tsinlingensis individuals with wet weights of 5.8 g, 12.3 g, 34.7 g, and 81.7 g, respectively, and the cycle threshold (Ct) values of B. lenok tsinlingensis in each tank were measured using quantitative PCR. Additionally, water samples were collected from six sites along the Zhouzhi Heihe River (Yu Dongquan, Ban Fangzi, Ba Mugou, two sites along Wang Jia, and Qingshui Rivers), and the Ct values of B. lenok tsinlingensis at these sites were quantified. The results show that the relationship between the Ct valu

SUBMITTER: Zhao H 

PROVIDER: S-EPMC11987999 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature