ALKBH5 Improves the Epithelial Cell Tight Junctions to Inhibit <i>Escherichia coli</i>-Induced Mastitis.

ABSTRACT: Mastitis poses a severe threat to the global cattle industry, causing huge economic losses. Environmental mastitis is mainly induced by Escherichia coli (E. coli), and the current treatment is still using antibiotics, with problems such as drug resistance and food safety. ALKBH5 is an RNA m⁶A demethylase that plays an important role in various biological processes, while p65 is a key regulator of inflammatory responses. Therefore, studying the interaction between ALKBH5 and p65 in protecting the mammary epithelial barrier provides new insights into the pathogenesis of mastitis. This study revealed that E. coli-induced acute inflammation activated the NF-κB/p65 signaling pathway and disrupted mammary epithelial cell tight junctions. Knockdown of ALKBH5 promot

SUBMITTER: Wu X 

PROVIDER: S-EPMC11988031 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature