Molecular Detection of SFGR in Ticks Collected from Yaks in Jiulong County of Sichuan Province, China.

ABSTRACT: This study investigated tick species and spotted fever group rickettsiae (SFGR) infection rates in ticks from yaks in Jiulong County, Sichuan Province, China. Firstly, ticks collected from yaks were meticulously classified through morphological identification. Subsequently, the total DNA of ticks was extracted, and specific partial sequences of their ITS-2 region, as well as the ompA and ompB genes of SFGR, were amplified using PCR. The positive PCR products were sequenced and compared thoroughly against the NCBI database. A phylogenetic analysis was conducted for ITS-2, ompA, and ompB to determine the species of tick and the SFGR. The results revealed that a total of 585 ticks were collected, with the most abundant species being R. microplus

SUBMITTER: Hao L 

PROVIDER: S-EPMC11988138 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature