MIRO1 Is Required for Dynamic Increases in Mitochondria-ER Contact Sites and Mitochondrial ATP During the Cell Cycle.

ABSTRACT: Mitochondria-ER contact sites (MERCS) are vital for mitochondrial dynamics, lipid exchange, Ca²⁺ homeostasis, and energy metabolism. We examined whether mitochondrial metabolism changes during the cell cycle depend on MERCS dynamics and are regulated by the outer mitochondrial protein mitochondrial rho GTPase 1 (MIRO1). Wound healing was assessed in mice with fibroblast-specific deletion of MIRO1. Wild-type and MIRO1^-/- fibroblasts and vascular smooth muscle cells were evaluated for proliferation, cell cycle progression, number of MERCS, distance, and protein composition throughout the cell cycle. Restoration of MIRO1 mutants was used to test the role of MIRO1 domains; Ca²⁺ transients and mitochondrial metabolism were evaluated using biochemical, immunodete

SUBMITTER: Endoni BT 

PROVIDER: S-EPMC11988184 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature