The LARP6 La module from <i>Tetrabaena socialis</i> reveals structural and functional differences from plant and animal LARP6 homologues.

ABSTRACT: This study identified the LARP6 La Module from Tetrabaena socialis (T. socialis), a four-celled green algae, in an effort to better understand the evolution of LARP6 structure and RNA-binding activity in multicellular eukaryotes. Using a combination of sequence alignments, domain boundary screens, and structural modelling, we recombinantly expressed and isolated the TsLARP6 La Module to > 98% purity for in vitro biochemical characterization. The La Module is stably folded and exerts minimal RNA binding activity against single-stranded homopolymeric RNAs. Surprisingly, it exhibits low micromolar binding affinity for the vertebrate LARP6 cognate ligand, a bulged-stem loop found in the 5'UTR of collagen type I mRNA, but does not bind double-stranded RNAs of similar

SUBMITTER: Lewis EM 

PROVIDER: S-EPMC11988235 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature