M⁶A-Modified GATA2 Enhances Odontogenic Differentiation in Stem Cells from the Apical Papilla.

ABSTRACT: Epigenetic modifications play a crucial role in regulating stem cell differentiation. Among these, N6-methyladenosine (m⁶A) modification significantly impacts mRNA stability and translation. However, its role in dental stem cell differentiation remains largely unexplored. Functional assays, including ALP activity, alizarin red S staining, qPCR, and Western blot, were conducted to assess odontogenic differentiation. Then, an in vivo dentin formation model was used to validate our findings. Additionally, we employed RNA stability assays and m⁶A site mutagenesis to investigate the regulatory mechanism of m⁶A modification in GATA2-mediated differentiation. Our results demonstrated that overexpression of GATA2 significantly promoted SCAP odontogenic differentiat

SUBMITTER: Yang H 

PROVIDER: S-EPMC11988903 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature