Chicken Primordial Germ Cells Do Not Proliferate in Insulin-Lacking Media.

ABSTRACT: Insulin is an important component of stem cell cultures; however, its role in the proliferation of avian primordial germ cells (PGCs) is unknown. The proliferation of PGCs in cultures varies and the growth factors and signaling pathways necessary to induce the proliferation of PGCs in chickens are unknown. Therefore, we conducted the present study to investigate the effect of insulin on the survival and proliferation of PGCs. In this study, we observed that under this culture system, PGCs proliferate in the presence of insulin, but do not proliferate in the absence of insulin. Furthermore, in insulin-lacking media, the expression of pluripotency genes, including LIN28, NANOG, POUV, and SOX2, was markedly decreased. Similarly, the expression of cell adhesion prot

SUBMITTER: Liu X 

PROVIDER: S-EPMC11988930 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature