Ex Vivo Plasma Application on Human Brain Microvascular Endothelial-like Cells for Blood-Brain Barrier Modeling.

ABSTRACT: hiPSC-derived blood-brain barrier (BBB) models are valuable for pharmacological and physiological studies, yet their translational potential is limited due to insufficient cell phenotypes and the neglection of the complex environment of the BBB. This study evaluates the plasma compatibility with hiPSC-derived microvascular endothelial-like cells to enhance the translational potential of in vitro BBB models. Therefore, plasma samples (sodium/lithium heparin, citrate, EDTA) and serum from healthy donors were tested on hiPSC-derived microvascular endothelial-like cells at concentrations of 100%, 75%, and 50%. After 24 h, cell viability parameters were assessed. The impact of heparin-anticoagulated plasmas was further evaluated regarding barrier function and endothelial phenotype of differenti

SUBMITTER: Nelz SC 

PROVIDER: S-EPMC11989380 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature