Optimized Protocol for High-Quality RNA Extraction from Grape Berry Skins Using Sorbitol Pre-Wash.

ABSTRACT: Extracting high-quality RNA from grape (Vitis Vinifera) berry skins is challenging due to their high levels of polysaccharides, phenolic compounds, sugars, and organic acids, which can negatively impact RNA purity and yield. Indeed, polyphenols can bind to RNA, polysaccharides may co-precipitate, and sugars and organic acids can interfere with the pH and ionic properties of the extraction buffer. Commercial kits offer a quick extraction method but are often ineffective for grape berry skins. Similarly, protocols that work well for other vegetal tissues are also inefficient and time-consuming for this tissue. To overcome these limitations, we optimized the RNA isolation by adding a sorbitol pre-wash step to both a non-commercial protocol and a commercial kit. Our results show that it

SUBMITTER: Prencipe A 

PROVIDER: S-EPMC11990153 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature