Phytochemical Analysis and In Vivo Anticancer Effect of <i>Becium grandiflorum</i>: Isolation and Characterization of a Promising Cytotoxic Diterpene.

ABSTRACT: Background:Becium grandiflorum is a fragrant perennial shrub of the Lamiaceae family. Objectives: The current study aimed to explore the cytotoxic potential of the n-hexane fraction from Becium grandiflorum aerial parts and, further, isolate its major diterpene and conduct in vitro and in vivo anticancer activities along with its molecular mechanism and synergy with doxorubicin. Methods: The hydroalcoholic extract of Becium grandiflorum aerial parts was fractionated, and the n-hexane fraction was analyzed via GC-MS. The major isolated diterpene, 18-epoxy-pimara-8(14),15-diene (epoxy-pimaradiene), was quantified using UPLC-PDA. Cytotoxicity assays were conducted on HCT-116, MCF-7, MDA-MB-231, and HepG2 cell lines. The synergistic effec

SUBMITTER: Fahim C 

PROVIDER: S-EPMC11990180 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature