A Protoplast System for CRISPR-Cas Ribonucleoprotein Delivery in <i>Pinus taeda</i> and <i>Abies fraseri</i>.

ABSTRACT: Climate change profoundly impacts the health, productivity, and resilience of forest ecosystems and threatens the sustainability of forest products and wood-based industries. Innovations to enhance tree growth, development, and adaptation offer unprecedented opportunities to strengthen ecosystem resilience and mitigate the effects of climate change. Here, we established a method for protoplast isolation, purification, and CRISPR-Cas ribonucleoprotein (RNP) delivery in Pinus taeda and Abies fraseri as a step towards accelerating the genetic improvement of these coniferous tree species. In this system, purified protoplasts could be isolated from somatic embryos with up to 2 × 10⁶ protoplasts/g of tissue and transfected with proteins and nucleotides, achieving deliver

SUBMITTER: Marques BM 

PROVIDER: S-EPMC11990275 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature