Developing a Multi-Method Approach for Understanding Cellular Uptake and Biological Response: Investigating Co-Exposure of Macrophage-like Differentiated THP-1 Cells to Al₂O₃ and CeO₂ Nanoparticles.

ABSTRACT: The use of different nanoparticles (NPs) is increasing in a wide variety of everyday products. Nevertheless, most studies concerning NP risk assessment have evaluated exposure scenarios involving a single kind of NP. A stepwise study distinguishing between the effects resulting from exposure to one kind of NP and those resulting from different co-exposure scenarios to Al2O3 and CeO2 NPs at concentrations below acute toxicity was conducted with different analytical techniques. As a starting point, WST-1 viability assays were performed to assess whether the chosen exposure concentrations resulted in any acute loss of viability, which would hamper further insight into the cellular response to NP exposure. Then, data on NP dissolution and uptake were obtained via single-particle inductively co

SUBMITTER: Hachenberger YU 

PROVIDER: S-EPMC11990466 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature