Transcriptional Dynamics Underlying Somatic Embryogenesis in <i>Coffea canephora</i>.

ABSTRACT: In Coffea canephora, a direct somatic embryogenesis (SE) protocol has been established by pretreating plants with NAA and kinetin, followed by the induction of leaf explants in a liquid medium with BA. Through a transcriptomic analysis of 10 key moments of the induction of SE in C. canephora, we were able to establish that the transcriptional responses of this process are divided into four stages. These stages correspond to the pretreatment, characterized by the positive regulation of genes associated with cell wall remodeling, flavonoid biosynthesis, and antioxidant activity that prepare the explants for the intense cellular activity that represents the induction of SE. During the first few hours, the early response to induction occurs, characterized by the highest number of

SUBMITTER: Couoh-Cauich MD 

PROVIDER: S-EPMC11991477 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature