Single cell transcriptome sequencing indicates the cellular heterogeneity of small intestine tissue in celiac disease.

ABSTRACT: Celiac disease (CeD) is an autoimmune small intestinal disease caused by gluten protein ingestion by genetically susceptible individuals. Genome-wide association studies and transcriptomic data have limited capacity to capture intercellular genetic variations. We aimed to construct a single cell transcriptome spectrum, analyze the immune microenvironment and cellular heterogeneity, discover disease-related specific genes and markers, and explore the pathogenesis of CeD. This study performed single cell RNA sequencing (scRNA-seq) on three small intestine biopsies from patients with CeD and three matched healthy Chinese controls. Immunohistochemistry (IHC) and quantitative polymerase chain reaction (qPCR) were used to validate potential diagnostic biomarkers of disease-differential genes. A

SUBMITTER: Shi T 

PROVIDER: S-EPMC11992159 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature