Cryopreserving 3D cell culture models of Alzheimer's disease in hydrogel microbeads.

ABSTRACT: Long-term preservation of fully differentiated human neurons poses a longstanding challenge in neuroscience research. Numerous cellular disease models have been established using cultured human neuronal cells, including our three-dimensional (3D) human neural cell culture model of Alzheimer's disease (AD). However, the absence of a reliable method for preserving fully differentiated human neural cell cultures for a long time has hindered the sharing and standardization of these models. To address this critical limitation, we focused on cryopreservation, which is the gold standard for long-term preservation, and combined this with three key technological advancements. First, we employed parallelized microfluidic devices for the efficient generation of 3D cell cultures within uniform hydroge

SUBMITTER: Kim JJ 

PROVIDER: S-EPMC11992178 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature