Diagnostic performance of allele-specific RT-qPCR and genomic sequencing in wastewater-based surveillance of SARS-CoV-2.

ABSTRACT: Clinical genomic surveillance is regarded as the gold standard for monitoring SARS-CoV-2 variants globally. However, as the pandemic wanes, reduced testing poses a risk to effectively tracking the trajectory of these variants within populations. Wastewater-based genomic surveillance that estimates variant frequency based on its defining set of alleles derived from clinical genomic surveillance has been successfully implemented. This method has its challenges, and allele-specific (AS) RT-qPCR or RT-dPCR may instead be used as a complementary method for estimating variant prevalence. Demonstrating equivalent performance of these methods is a prerequisite for their continued application in current and future pandemics. Here, we compared single-allele frequency using AS-RT-qPCR, to single-alle

SUBMITTER: Kabir MP 

PROVIDER: S-EPMC11992540 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature