Role of SerpinA3 in the Pathogenesis of Graves' Orbitopathy in Orbital Fibroblasts.

ABSTRACT:

Purpose

We investigated the implications of SerpinA3, a secretory serine protease inhibitor, in inflammation and adipogenesis of Graves' orbitopathy (GO). To identify its precise function in GO pathogenesis, we evaluated the role of SerpinA3 in the inflammation and adipogenesis of GO.

Methods

SerpinA3 expression was compared between GO (n = 30) and normal participants (n = 28) in orbital tissue explants using real-time PCR. Orbital fibroblasts from GO (n = 3) and normal participants (n = 3) were transfected with or without small interfering RNA against SerpinA3 before IL-1β stimulation. Western blotting assessed inflammatory cytokine and signaling molecule expression. Adipogenic differentiation was assessed using Oil Red O staining, and adipogenic marker expression was deter

SUBMITTER: Kim MS 

PROVIDER: S-EPMC11993136 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature