Single-Cell Profiling of Tubular Epithelial Cells in Adaptive State in the Urine Sediment of Patients With Early and Advanced Diabetic Kidney Disease.

ABSTRACT:

Introduction

Tubular epithelial cells (TECs) under adaptive state (aTECs) have been frequently reported in the injured kidney closely associated with disease progression. However, whether aTECs is present in the urine of patients with diabetic kidney disease (DKD) and its clinical implication have not been assessed.

Methods

Urine samples from patients with early and advanced DKD were collected and subjected to single-cell RNA sequencing (scRNA-seq). Kidney single nucleus RNA-seq, spatial scRNA-seq, and bulk RNA-seq datasets were employed to reconstruct their local environment and to delineate differences between shed cells and local residence.

Results

Most urinary TEC in patients with DKD are under adaptive states. Whereas the composition of urinary TEC is consistent

SUBMITTER: Ma D 

PROVIDER: S-EPMC11993230 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature