Disulfiram ameliorates bone loss in ovariectomized mice by suppressing osteoclastogenesis.

ABSTRACT:

Introduction

Disulfiram (DSF), known as an anti-alcoholism drug, has been reported to suppress osteoclast differentiation in vitro; however, it remains uncertain whether DSF is effective in preventing osteoclastogenesis in vivo. This study aimed to investigate the effect of DSF administration in osteoporotic mice and its contribution to osteoclastogenesis in vivo.

Materials and methods

The bone phenotype of ovariectomized mice, both treated and untreated with DSF, was examined using microcomputed tomography analysis. Osteoclastic and osteoblastic parameters were assessed through bone morphometric analysis. The direct effect of DSF on osteoblastogenesis in vitro was evaluated via a primary osteoblast culture experiment. The expression of genes related to DSF targets (Nup85, C

SUBMITTER: Fukui T 

PROVIDER: S-EPMC11993463 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature