Single-cell RNA sequencing characterization of Holstein cattle blood and milk immune cells during a chronic Staphylococcus aureus mastitis infection.

ABSTRACT: Mastitis remains the most prevalent and costly disease to dairy producers. Granulocytes are the primary host innate immune cell responders during infectious mastitis. Here we examine three mid-lactation Holsteins challenged with ~ 150 CFU of Staphylococcus aureus (Newbould) that developed chronic mastitis as assessed by bacteria and somatic cell counts in a single quarter. Single-cell RNA-sequencing (scRNA-seq) of blood and milk cells identified immune cell populations of interest from both tissues, and the proportion of cell types recovered via scRNA-seq were highly similar to those recovered via flow cytometry. Granulocytes were the predominating cell type in both blood and milk samples; however granulocytes identified via scRNA-seq revealed several clusters comprised primarily of milk-d

SUBMITTER: Wiarda JE 

PROVIDER: S-EPMC11993596 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature