Molecular identification of somatostatin family in tilapia and the effect of food intake on pss2 and sstr3a.

ABSTRACT: Somatostatin (SS) plays an important role in regulating food intake and following digestive functions of vertebrates mediated via somatostatin receptor (SSTR). However, the feedback regulation by which food intake regulate SS and SSTRs expression, and thereby their functions, remains poorly understood. Here, we cloned somatostatin genes (pss1, pss2 and pss3) and somatostatin receptors genes (sstr2a, sstr2b, sstr2c, sstr3a, sstr3b, sstr5a and sstr5b) from tilapia, Oreochromis niloticus and conducted their tissue distribution. Phylogenetic analysis and genomic synteny maps revealed their high homology across the vertebrates and evolution history. Following, synthesized SS-14 and SS-28 were both confirmed to bind to SSTRs and mediated Erk1/2 or Akt phosphorylation in a dose-dependent manner a

SUBMITTER: Cai R 

PROVIDER: S-EPMC11993745 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature