Quality control maps: Real-time quantitative control of single-molecule localization microscopy data.

ABSTRACT: Single-molecule localization microscopy (SMLM) has revolutionized the understanding of cellular organization by reconstructing informative images with quantifiable spatial distributions of molecules far beyond the optical diffraction limit. Much effort has been devoted to optimizing localization accuracy. One such approach is the assessment of SMLM data quality in real time rather than after lengthy postacquisition analysis, which nevertheless represents a computational challenge We overcame this difficulty by implementing an innovative mathematical approach we designed to drastically reduce the computational analysis of particle localization. Our quality control maps (QCM) workflow enables a much higher rate of data processing compared to that limited by the frequency required by current

SUBMITTER: Mailfert S 

PROVIDER: S-EPMC11993929 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature