Small molecules direct the generation of ameloblast-like cells from human embryonic stem cells.

ABSTRACT:

Background

Ameloblasts present a promising avenue for the investigation of enamel and tooth regeneration. Previous protocols for directing the differentiation of human embryonic stem cells (hESCs) into dental epithelial (DE) cells involving the need for additional cells, conditional medium, and the use of costly cytokines. Importantly, ameloblasts have not been generated from hESCs in previous studies. Hence, we aimed to identify defined differentiation conditions that would solely utilize small molecules to achieve the production of ameloblasts.

Methods

We developed a three-step strategy entailing the progression of hESCs through non-neural ectoderm (NNE) and DE to generate functional ameloblasts in vitro. Initially, the NNE fate was induced from hESCs using a 6-day differe

SUBMITTER: Zhu X 

PROVIDER: S-EPMC11993985 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature