The role of transketolase in the immunotherapy and prognosis of hepatocellular carcinoma: a multi-omics approach.

ABSTRACT:

Objective

To explore the role of transketolase (TKT) in the immunotherapy and prognosis of hepatocellular carcinoma (HCC).

Materials and methods

TKT expression across various cancers and its associations with tumor immunity and prognosis were analyzed using nomogram models. A multi-omics approach was employed, including bulk RNA-seq analysis, methylation profiling, single-cell analysis, and spatial transcriptomics. Experimental methods included RT-qPCR, siRNA transfection, luciferase reporter assay, and chromatin immunoprecipitation.

Results

TKT was significantly upregulated in multiple cancers and correlated with immune cell infiltration, particularly in HCC. Elevated TKT expression was associated with poor overall survival (OS) in HCC and was an independent prognost

SUBMITTER: Gu XY 

PROVIDER: S-EPMC11994433 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature