Suitable concentrations of brassinolide application enhances growth performance and saikosaponin biosynthesis by transcriptional activation of triterpenoid pathway genes in <i>Bupleurum chinense</i> DC.

ABSTRACT: The role of brassinolides (BRs) in regulating the synthesis of plant secondary metabolites has been recognized. However, the effect of brassinolides on the synthesis of saikosaponin in Bupleurum chinense DC. (B. chinense) is still unresolved, To address this knowledge gap, experiments were conducted in which different concentrations (0 mg/L as CK, 0.1 mg/L, 0.2 mg/L, and 0.4 mg/L) of BRs solution were sprayed on B. chinense taproot in the present study. We measured the growth indicators of each group of B. chinense, used quantitative real-time PCR (qRT-PCR) to determine the expression level of genes related to the biosynthesis of saikosaponin, used terpenoid-targeted metabolomics to determine the accumulation of saikosaponin, and verified the metabolomics result

SUBMITTER: Zhou S 

PROVIDER: S-EPMC11994733 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature