A semi-automated observation approach to quantify mouse skeletal muscle differentiation using immunohistochemistry.

ABSTRACT: Histological analysis is vital for understanding skeletal muscle diseases. However, quantifying data requires much effort, so automation is expected to reduce workload. The present study proposes a semi-automated method to quantify expressed paired box protein (Pax-7) /bromodeoxyuridine (BrdU)-positive cells. Soleus muscle was harvested from mice 2 weeks after oral administration of the epicatechin tetramer cinnamantanin A2 (A2), known to induce skeletal muscle hypertrophy. Before the necropsy, mice were treated with BrdU to facilitate cell tracking. For histological examination, frozen sections were stained with hematoxylin and eosin (HE) to measure cell size by cross-sectional area (CSA) and were immunostained with anti-BrdU and anti-Pax-7 antibodies. Treatment with A2 caused a shift in

SUBMITTER: Shimizu K 

PROVIDER: S-EPMC11994891 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature