α- and β-myosin II can be non-uniformly distributed within the cardiac sarcomere.

ABSTRACT: In humans, the forces of the heartbeat are generated by both "fast" α-myosin II and "slow" β-myosin II molecular motors. Here, we report that α-myosin II and β-myosin II can take on an anisotropic arrangement within cardiac sarcomeres, the minimal contractile unit of cardiac muscle. In induced pluripotent stem cell (iPSC)-derived cardiac myocytes, we find that α-myosin II is distributed primarily within the central region of the filaments, being mostly absent from the distal ends, whereas β-myosin II is distributed along the entire filament. We measure that the outer bounds of α-myosin II in filament stacks are shorter than those of β-myosin II in both the zebrafish embryo and adult human myocardium. Our findings suggest that the two motors that drive the human heartbeat can have a specifi

SUBMITTER: Hayes JB 

PROVIDER: S-EPMC11994913 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature