Specificity landscapes of 40 R2R3-MYBs reveal how paralogs target different <i>cis</i>-elements by homodimeric binding.

ABSTRACT: Paralogous transcription factors (TFs) frequently recognize highly similar DNA motifs. Homodimerization can help distinguish them according to their different dimeric configurations. Here, by studying R2R3-MYB TFs, we show that homodimerization can also directly change the recognized DNA motifs to distinguish between similar TFs. By high-throughput SELEX, we profiled the specificity landscape for 40 R2R3-MYBs of subfamily VIII and curated 833 motif models. The dimeric models show that homodimeric binding has evoked specificity changes for AtMYBs. Focusing on AtMYB2 as an example, we show that homodimerization has modified its specificity and allowed it to recognize additional cis-regulatory sequences that are different from the closely related CCWAA-box AtMYBs and are unique among a

SUBMITTER: Li T 

PROVIDER: S-EPMC11995187 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature