Enhancement of gut barrier integrity by a <i>Bacillus subtilis</i> secreted metabolite through the GADD45A-Wnt/β-catenin pathway.

ABSTRACT: Inflammatory bowel disease (IBD) represents a significant challenge to global health, characterized by intestinal inflammation, impaired barrier function, and dysbiosis, with limited therapeutic options. In this study, we isolated a novel strain of Bacillus subtilis (B. subtilis) and observed promising effects in protecting against disruption of the gut barrier. Our findings indicate that the enhancement of intestinal barrier function is primarily attributed to its metabolites. We identified a novel metabolite, 2-hydroxy-4-methylpentanoic acid (HMP), derived from B. subtilis, that significantly improved intestinal barrier function. We also show that growth arrest and DNA damage 45A (GADD45A) is a key regulator of mucosal barrier integrity, which is activated by HMP and

SUBMITTER: Liu S 

PROVIDER: S-EPMC11995189 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature