Efficient isothermal expansion of human telomeric and minisatellite repeats by Thermococcus litoralis DNA polymerase.

ABSTRACT: Repeating DNA sequences, such as telomeres, centromeres, and micro- and mini-satellites, comprise 50% of the genome and play important roles in regulatory and pathogenic mechanisms. In order to study structures and functions of such repeating sequences, it is important to have simple and efficient methods for making them in vitro. Here, we describe the efficient and convenient expansion of repetitive telomeric and minisatellite DNA sequences starting from small synthetic templates to final product lengths of several hundreds to thousands of nucleotides by the thermostable DNA polymerase from Thermococcus litoralis (Vent DNA polymerase). This enzyme was so far unknown to catalyze repeat expansion. Either single-stranded or double-stranded DNAs could be produced, depending on nucleotides pre

SUBMITTER: Hartig JS 

PROVIDER: S-EPMC1199558 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature