Proteome of granulosa cells lipid droplets reveals mechanisms regulating lipid metabolism at hierarchical and pre-hierarchical follicle in goose.

ABSTRACT: Avian hierarchical follicles are formed by selection and dominance of pre-hierarchical follicles, and lipid metabolism plays a pivotal role in this process. The amount of lipid in goose follicular granulosa cells increases with the increase of culture time, and the neutral lipid in the cells is stored in the form of lipid droplets (LDs). LD-associated proteins (LDAPs) collaborate with LDs to regulate intracellular lipid homeostasis, which subsequently influences avian follicle development. The mechanism by which LDAPs regulate lipid metabolism in goose granulosa cells at different developmental stages is unclear. Therefore, using BODIPY staining, we found that at five time points during in vitro culture, the LD content in hierarchical granulosa cells was significantly higher than th

SUBMITTER: Yuan X 

PROVIDER: S-EPMC11995638 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature