Phytochemical production and antioxidant activity improvement of <i>Rhinacanthus nasutus</i> (L.) Kurz calli by <i>in vitro</i> polyploidization.

ABSTRACT:

Background

Rhinacanthus nasutus (L.) Kurz is a multipurpose ethnomedicinal shrub containing various bioactive compounds and phytochemicals. Inducing polyploidy is an alternative way to enhance the production of secondary metabolites in medicinal plants. The main objectives of this research were to study the effect of polyploidization on the phytochemical content and antioxidant activity of R. nasutus calli.

Methods

In vitro polyploidy was induced by soaking calli in colchicine at different concentrations and for different exposure times. To determine callus polyploidy, the relative DNA contents of each sample were examined using flow cytometry. Diploid, tetraploid, and mixoploid calli were extracted to determine the total phenolic content (TPC), total fl

SUBMITTER: Yaowachai W 

PROVIDER: S-EPMC11995891 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature