A systematic search for new mammalian noncoding RNAs indicates little conserved intergenic transcription.

ABSTRACT:

Background

Systematic identification and functional characterization of novel types of noncoding (nc)RNA in genomes is more difficult than it is for protein coding mRNAs, since ncRNAs typically do not possess sequence features such as splicing or translation signals, or long open reading frames. Recent "tiling" microarray studies have reported that a surprisingly larger proportion of mammalian genomes is transcribed than was previously anticipated. However, these non-genic transcripts often appear to be low in abundance, and their functional significance is not known.

Results

To systematically search for functional ncRNAs, we designed microarrays to detect 3,478 intergenic and intronic sequences that are conserved between the human, mouse, and rat genomes, and that score hig

SUBMITTER: Babak T 

PROVIDER: S-EPMC1199595 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature