Overexpression of tRNA m⁷G modification methyltransferase complex promotes the biosynthesis of triterpene in yeast.

ABSTRACT:

Background

The sustainable production of valuable compounds using microbial cell factories is an effective approach, yet further metabolic engineering strategies are needed to enhance their biosynthetic potential. Recent studies suggest that RNA modifications can influence cellular metabolism, but their role in metabolic engineering remains largely unexplored.

Methods

The production of squalene and lupeol in different yeast strains was detected by gas chromatography-mass spectrometry (GC-MS) equipment. Transcriptomic analysis was performed to identify metabolic changes associated with the epigenetic modification. The transcriptional and translational expression of targeted genes were determined by real-time quantitative polymerase chain reaction and western blotting, respect

SUBMITTER: Ma M 

PROVIDER: S-EPMC11996079 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature