Bayesian analysis and efficient algorithms for single-molecule fluorescence data and step counting.

ABSTRACT: With the growing adoption of single-molecule fluorescence experiments, there is an increasing demand for efficient statistical methodologies and accurate analysis of the acquired measurements. Existing analysis frameworks, such as those that use kinetic models, often rely on strong assumptions on the dynamics of the molecules and fluorophores under study that render them inappropriate for general purpose step counting applications, especially when the systems of study exhibit uncharacterized dynamics. Here, we propose a novel Bayesian nonparametric framework to analyze single-molecule fluorescence data that is kinetic model independent. For the evaluation of our methods, we develop four MCMC samplers, ranging from elemental to highly sophisticated, and demonstrate that the added complexity

SUBMITTER: Mattamira C 

PROVIDER: S-EPMC11996346 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature