Large adipocytes alter mode of lipid release and promote breast cancer malignancy.

ABSTRACT: Primary adipocytes possess a dramatic capacity to expand and retract in volume, leading to high variability in cell size within and between individuals. Yet, how adipocyte size impacts cell function remains unclear as adipocyte size is not tunable with traditional experimental approaches, forcing previous work to rely on correlative studies. Here, we develop protocols to separate primary adipocytes from the same donor into large and small populations and maintain these size-sorted cells in culture. Using these methods, we perform transcriptomic, lipidomic, and functional analyses on large and small adipocytes across two orthogonal mouse models of obesity and validate our results with human clinical samples. Our findings indicate that changes to cell size, rather than global differences med

SUBMITTER: Beeghly GF 

PROVIDER: S-EPMC11996363 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature