Factors Influencing Fluorescence-activated Cell Sorting for Multiple Myeloma Fluorescence <i>in situ</i> Hybridization: Real-world Experience.

ABSTRACT:

Background

FISH is the standard method for detecting cytogenetic abnormalities (CAs) in patients with multiple myeloma, and pre-enrichment of plasma cells is recommended to increase detection rates. However, optimal strategies to ensure sufficient plasma cell retrieval when standard enrichment techniques fail remain underexplored. We investigated factors influencing the success of fluorescence-activated cell sorting (FACS) and assessed the use of direct FISH in cases in which FACS failed.

Methods

A retrospective analysis was conducted on 457 bone marrow samples submitted for FISH between November 2016 and May 2022. FACS was considered successful when plasma cells (CD38+ and CD138+ cells) constituted >1% of the total number of cells. Direct FISH was performed for samples with

SUBMITTER: Choi J 

PROVIDER: S-EPMC11996684 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature