Establishment of a Multilocus Sequence Typing Scheme for <i>Pasteurella canis</i> Using Isolates from Infected Humans and Diseased Companion Animals.

ABSTRACT:

Background

Multilocus sequence typing (MLST) is well-established for Pasteurella multocida but remains undeveloped for Pasteurella canis. We established MLST for P. canis using isolates from humans and companion animals in Japan and Korea to gain insights into its population biology.

Methods

We analyzed 39 and 22 isolates from companion animals and humans, respectively. We selected seven housekeeping genes-adk, aroA, deoD, gdhA, g6pd, mdh, and pgi-used in P. multocida MLST. Primer pairs for PCR amplification and sequencing were designed based on conserved sites in 10 whole-genome sequences. We determined fragment sequences, variable sites, allelic profiles, and sequence types (STs) of each isolate. A phylogenetic tree of concatenated

SUBMITTER: Yoshida H 

PROVIDER: S-EPMC11996689 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature