A simplified preparation method for single-nucleus RNA-sequencing using long-term frozen brain tumor tissues.

ABSTRACT: Single-cell RNA-sequencing has provided intriguing new insights into research areas such as developmental processes and tumor heterogeneity. Most approaches, however, rely on the availability of fresh surgical specimens, thereby dramatically reducing the ability to profile particularly rare tissue types. Here, we optimized a method to isolate intact nuclei from long-term frozen pediatric glioma tissues. We performed a technical comparison between different single-nucleus RNA-sequencing (snRNA-seq) systems and applied the established nucleus isolation method to analyze frozen primary glioma tissues. The results show that our fast, simple and low-cost nuclear isolation protocol provides intact nuclei, which can be used in both droplet- and plate-based single-cell sequencing platforms - allow

SUBMITTER: Ernst KJ 

PROVIDER: S-EPMC11997191 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature