High dose acetaminophen re-polarizes CD11b+ cells in the tumor microenvironment towards an activated macrophage phenotype.

ABSTRACT:

Objective

High dose acetaminophen (AAP) has demonstrated promising results in early phase clinical trials for treatment of advanced malignancies. When administered concurrently with the CYP2E1 inhibitor fomepizole, AAP can be dose-escalated 100-fold relative to standard dosing without liver toxicity and without compromising anti-tumor activity.

Methods and analysis

The current study used a 23-plex flow cytometry panel to study AAP-induced changes in the tumor immune microenvironment of syngeneic mouse breast tumors. Effects of AAP on macrophage function and antigen presentation, alone and in combination with PD-1 antibodies, were evaluated.

Results

Findings demonstrated that the vast majority of CD45+ cells in the triple negative breast cancer model are CD11b+ cells o

SUBMITTER: Bryan A 

PROVIDER: S-EPMC11997351 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature