YibN, a bona fide interactor of the bacterial YidC insertase with effects on membrane protein insertion and membrane lipid production.

ABSTRACT: YidC, a prominent member of the Oxa1 superfamily, is essential for the biogenesis of the bacterial inner membrane, significantly influencing its protein composition and lipid organization. It interacts with the Sec translocon, aiding the proper folding of multi-pass membrane proteins. It also functions independently, serving as an insertase and lipid scramblase, augmenting the insertion of smaller membrane proteins while contributing to the organization of the bilayer. Despite the wealth of structural and biochemical data available, how YidC operates remains unclear. To investigate this, we employed proximity-dependent biotin labeling (BioID) in Escherichia coli, leading to the identification of YibN as a crucial component within the YidC protein environment. We then demonstrated the assoc

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC11997395 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature