Identification of host specificity determinants in brome mosaic virus for rice infection.

ABSTRACT: Brome mosaic virus (BMV) is a tripartite positive-stranded RNA plant virus. The genomic RNA2 encodes the 2a protein, which has conserved RNA-dependent RNA polymerase motifs and is required for viral RNA replication. In this study, we have used two BMV strains, F and KU5, and identified two key amino acid residues, 776R and 784T, in the C-terminal non-conserved region of the 2a protein that are critical for systemic infection of BMV-F in rice. While KU5 strain was not able to systemically infect rice, the KU5 mutant strain with two codon changes for 776R and 784T in the 2a gene gained the ability to establish systemic infection in rice, which affects long-distance movement, but not replication or cell-to-cell movement. Through infection assays of KU5 synonymous mutant strains, we demonstrat

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11997414 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature