Comparative genomic and phenotypic description of <i>Escherichia ruysiae</i>: a newly identified member of the gut microbiome of the domestic dog.

ABSTRACT:

Introduction

Escherichia ruysiae is a newly identified species within the Escherichia genus, yet its presence in domestic animals remains largely unexamined. This study characterizes four isolates detected for the first time in the domestic dog (Canis lupus familiaris), focusing on their phenotypic and genomic features.

Methods

We used culturomic methods to isolate four E. ruysiae isolates that were initially identified as Escherichia coli using MALDI-TOF mass spectrometry. Whole-genome sequencing confirmed that the isolates belonged to E. ruysiae, not E. coli. Phenotypic characterization included enzymatic activity assays and antimicrobial susceptibility testing. Comparative genomic analyses were performed on these four isolates, al

SUBMITTER: Dione N 

PROVIDER: S-EPMC11997573 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature