Transcriptional induction by ecdysone in Drosophila salivary glands involves an increase in chromatin accessibility and acetylation.

ABSTRACT: Transcriptional activation by 20-hydroxyecdysone (20E) in Drosophila provides an excellent model for studying tissue-specific responses to steroids. An increase in the 20E concentration regulates the degradation of larval and the proliferation of adult tissues during metamorphosis. To study 20E-dependent transcription, we used the natural system for controlling the 20E concentration-the E23 membrane transporter-which exports 20E from the cell. We artificially expressed E23 in tissues to suppress the first wave of 20E-inducible transcription at metamorphosis. E23 expression revealed a plethora of 20E-dependent genes in salivary glands, while mildly affecting transcription in brain. We described the mechanisms controlling transcriptional activation by 20E in salivary glands. 20E depletion de

SUBMITTER: Evdokimova AA 

PROVIDER: S-EPMC11997763 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature