Autophagy induction enhances homologous recombination-associated CRISPR-Cas9 gene editing.

ABSTRACT: CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 (CRISPR-associated protein 9)-based gene editing via homologous recombination (HR) enables precise gene correction and insertion. However, its low efficiency poses a challenge due to the predominance of nonhomologous end-joining during DNA repair processes. Although numerous efforts have been made to boost HR efficiency, there remains a critical need to devise a novel method that can be universally applied across cell types and in vivo animals, which could ultimately facilitate therapeutic treatments. This study demonstrated that autophagy induction using different protocols, including nutrient deprivation or chemical treatment, significantly improved HR-associated gene editing at diverse genomic loci in mammalian cell

SUBMITTER: Nam HJ 

PROVIDER: S-EPMC11997770 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature