High glucose induces senescence in synovial mesenchymal stem cells through mitochondrial dysfunction.

ABSTRACT:

Purpose

To investigate the impact of high glucose on the senescence of synovial mesenchymal stem cells (SMSCs) and to elucidate the role of mitochondrial dysfunction in this process.

Methods

‌SMSCs were treated with medium containing high glucose (25 mmol/L) or low glucose (5.5 mmol/L) concentrations. The effects of high glucose concentrations on the proliferation, senescence, mitochondrial reactive oxygen species (ROS) levels, mitochondrial fission, and mitophagy of SMSCs were investigated. First, the impact of 24-hour high glucose treatment on SMSCs was investigated‌. After this initial 24-hour exposure, the medium was subsequently changed to low glucose, and the cells were cultivated for an additional 24 h; this was then compared with the effects of continuous 48-hour hig

SUBMITTER: Tan S 

PROVIDER: S-EPMC11998213 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature